DNA Molar Concentration Calculator

DNA Molar Concentration Formula:

\[ M = \frac{\mu g/mL \times 10^{-3}}{660 \times bp\ length} \]

DNA Molar Concentration (M):

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1. What Is DNA Molar Concentration?

DNA molar concentration refers to the number of moles of DNA per liter of solution. It is an important measurement in molecular biology for various applications such as PCR, sequencing, and cloning experiments where precise DNA quantities are required.

2. How Does The Calculator Work?

The calculator uses the DNA molar concentration formula:

\[ M = \frac{\mu g/mL \times 10^{-3}}{660 \times bp\ length} \]

Where:

\( M \) — Molar concentration (mol/L)
\( \mu g/mL \) — DNA concentration in micrograms per milliliter
\( 660 \) — Average molecular weight per base pair (g/mol)
\( bp\ length \) — DNA length in base pairs

Explanation: The formula converts mass concentration to molar concentration by accounting for the molecular weight of DNA, which averages 660 g/mol per base pair.

3. Importance Of DNA Molar Concentration

Details: Accurate DNA molar concentration calculation is essential for molecular biology experiments where precise stoichiometry is required, such as in PCR setup, restriction enzyme digestion, ligation reactions, and sequencing library preparation.

4. Using The Calculator

Tips: Enter DNA concentration in μg/mL and DNA length in base pairs. Both values must be positive numbers. The calculator will provide the molar concentration in mol/L.

5. Frequently Asked Questions (FAQ)

Q1: Why is 660 g/mol used as the average molecular weight?
A: 660 g/mol represents the average molecular weight of one DNA base pair, accounting for variations between nucleotide pairs (A-T: 615 g/mol, G-C: 616 g/mol).

Q2: Can this calculator be used for RNA concentration?
A: No, this calculator is specific for double-stranded DNA. For RNA calculations, use 340 g/mol as the average molecular weight per nucleotide.

Q3: How accurate is this calculation for short oligonucleotides?
A: The calculation works well for oligonucleotides longer than 15 base pairs. For very short sequences, the exact molecular weight should be calculated based on the specific nucleotide sequence.

Q4: What if my DNA is single-stranded?
A: For single-stranded DNA, use 330 g/mol as the average molecular weight per nucleotide instead of 660 g/mol per base pair.

Q5: Why is molar concentration important in molecular biology?
A: Molar concentration allows researchers to work with precise molecular ratios, which is critical for reactions like PCR, restriction digests, and ligations where the stoichiometry of components affects the outcome.