Ng To Nmol Calculator Illumina

Illumina Oligo Conversion Formula:

\[ nmol = \frac{ng}{Molar\ Mass} \times 1000 \]

Nanomoles (nmol):

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1. What Is The Ng To Nmol Calculator Illumina?

The Ng To Nmol Calculator Illumina is a specialized tool for converting oligonucleotide mass (ng) to molar quantity (nmol) using Illumina's specific conversion formula. This is particularly useful for Illumina sequencing workflows and oligo normalization.

2. How Does The Calculator Work?

The calculator uses the Illumina conversion formula:

\[ nmol = \frac{ng}{Molar\ Mass} \times 1000 \]

Where:

\( ng \) — Mass of oligonucleotide in nanograms
\( Molar\ Mass \) — Molecular weight of the oligo in g/mol
\( 1000 \) — Conversion factor from µmol to nmol

Explanation: The formula converts mass-based measurements to molar quantities, which is essential for accurate oligo normalization in Illumina sequencing protocols.

3. Importance Of Oligo Concentration Conversion

Details: Accurate conversion between mass and molar units is crucial for Illumina library preparation, ensuring consistent oligo concentrations and optimal sequencing performance.

4. Using The Calculator

Tips: Enter the oligo mass in nanograms (ng) and the molar mass in g/mol. Both values must be positive numbers. The calculator will provide the result in nanomoles (nmol).

5. Frequently Asked Questions (FAQ)

Q1: Why is this conversion important for Illumina workflows?
A: Illumina sequencing requires precise molar concentrations of oligos for library preparation and normalization to ensure even sequencing coverage.

Q2: How do I determine the molar mass of my oligo?
A: The molar mass can be calculated using online oligo calculators or provided by the manufacturer. It's typically around 6000-7000 g/mol for standard Illumina adapters.

Q3: Can this calculator be used for other applications?
A: While designed for Illumina oligos, the formula can be applied to any oligonucleotide conversion from mass to molar units.

Q4: What precision should I expect from this calculation?
A: The calculator provides results with 4 decimal places, which is sufficient for most molecular biology applications.

Q5: Are there any limitations to this conversion?
A: The accuracy depends on the precision of your mass measurement and the correctness of the molar mass value used in the calculation.