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Protein Molar Concentration Formula:
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Protein molar concentration refers to the number of moles of protein per liter of solution, expressed in mol/L. It's a fundamental measurement in biochemistry and molecular biology for quantifying protein solutions.
The calculator uses the molar concentration formula:
Where:
Explanation: The formula converts mass concentration (mg/mL) to molar concentration (mol/L) using the molecular weight of the protein.
Details: Accurate molar concentration is essential for experimental consistency, protein-protein interaction studies, enzyme kinetics, and preparing solutions with precise stoichiometry.
Tips: Enter protein concentration in mg/mL and molecular weight in g/mol. Both values must be positive numbers. The calculator will provide the molar concentration in mol/L.
Q1: Why convert mg/mL to molar concentration?
A: Molar concentration allows for comparison of molecules based on the number of molecules rather than mass, which is crucial for stoichiometric calculations and biological activity assessments.
Q2: How do I determine protein molecular weight?
A: Molecular weight can be calculated from the amino acid sequence, measured by mass spectrometry, or obtained from protein databases and literature.
Q3: What is a typical molar concentration range for protein solutions?
A: This varies widely depending on the protein and application, but common working concentrations range from nanomolar (10⁻⁹ M) to millimolar (10⁻³ M) levels.
Q4: Does this calculation account for protein purity?
A: No, this calculation assumes 100% pure protein. For impure samples, the actual molar concentration will be lower than calculated.
Q5: Can this calculator be used for other biomolecules?
A: Yes, the same formula applies to any molecule when you know its molecular weight and mass concentration.